The Form I-526E filing fee and the later I-829 fee from the USCIS fee schedule, immigrant-visa or adjustment fees for each family member, medical examinations, and the regional center's own administrative fee, which is separate from the invested capital and is not at risk in the same way.
Keep the capital, the government fees and the project fee in three columns
The EB-5 investment amount — US$1,050,000, or US$800,000 in a targeted employment area or infrastructure project, subject to statutory adjustment — is capital placed at risk in the new commercial enterprise. Around it sit three other categories of cost that should never be blended into one figure. Government fees are set by USCIS on Form G-1055 and by the State Department for immigrant visas: the I-526E petition fee, the I-829 fee at the end of the conditional period, visa application fees for each family member if processing abroad, and the immigrant fee on entry.
Regional centers charge an administrative or subscription fee on top of the investment; it pays for project management and is ordinarily not returned, so read the offering documents for how it is treated. Professional costs for the source-of-funds work, translations of French-language farm records and any accounting reconstruction form the third column. A family should be able to state each column separately before signing, and should confirm with the project which payments are refundable if the petition is refused.